The effects of NACL, KCL and MGCL2 on the germination of Brassica rapa var. parachinensis seed

Authors

  • Mona Ghanad

    universiti putra malaysia
  • Rosimah Binit Nulit

  • Rusea Go

  • Christina Yong Seok Yien

How to Cite

Ghanad, M., Binit Nulit, R., Go, R., & Yong Seok Yien, C. (2016). The effects of NACL, KCL and MGCL2 on the germination of Brassica rapa var. parachinensis seed. International Journal of Biological Research, 4(1), 52-55. https://doi.org/10.14419/ijbr.v4i1.5854

Received date: February 9, 2016

Accepted date: March 1, 2016

Published date: April 19, 2016

DOI:

https://doi.org/10.14419/ijbr.v4i1.5854

Keywords:

Brassica, Germination Percentage, Salt Tolerance, Seed Vigor.

Abstract

This study was conducted to determine seed germination on the mechanisms of salinity stress in B. rapa var. parachinensis (Hong kong choy sum). The type of experiment was a completely randomized design with three salinity treatment NaCl, MgCl2, and KCl and minimum four replications was used. Salinity treatment consisted of 0 (control), 50, 100, 150, 200, mM concentrations. The experiment was carried out under 70% relative humidity at 23°C with a photoperiod of 8:16 h light/dark. The germinated seeds were counted every day and germination percentage, seed vigor, seed tolerance, length of seedling and biomass seedling was measured. The data were analyzed by one-way ANOVA multiple comparison test followed by Duncan’s multiple range test.

References

  1. [1] Abdul-Baki, A. A., & Anderson, J. D. (1973) Vigor determination in soybean seed by multiple criteria. Crop science 13, 630-633. http://dx.doi.org/10.2135/cropsci1973.0011183X001300060013x.

    [2] Association of Official Seed Analysts. (2002). Seed vigor testing handbook from http://www.aosaseed.com/ seed vigor testing handbook.

    [3] Bouaziz, A., & Hicks, D. (1990) Consumption of wheat seed reserves during germination and early growth as affected by soil water potential. Plant and soil 128, 161-165. http://dx.doi.org/10.1007/BF00011105.

    [4] Brandenburg, W., & Kleier, C. (2011) Effect of MgCl2 on germination, growth and biomass allocation of the radish cv.“cherry belleâ€. American Journal Environmental Science 7, 132-135. http://dx.doi.org/10.3844/ajessp.2011.132.135.

    [5] Bybordi and Tabatabaei, J. (2009) Oxidative Stress and Neurodgenerative Diseases: A review of upstream and downstream antioxidant therapeutic options. Current Neurophacology 7, 65-74. http://dx.doi.org/10.2174/157015909787602823.

    [6] Cuin, T. A., Bose, J., Stefano, G., Jha, D., Tester, M., Mancuso, S., & Shabala, S. (2011) Assessing the role of root plasma membrane and tonoplast Na+/H+ exchangers in salinity tolerance in wheat: in planta quantification methods. Plant, Cell & Environment 34, 947-961. http://dx.doi.org/10.1111/j.1365-3040.2011.02296.x.

    [7] Deng, Y., Yuan, F., Feng, Z., Ding, T., Song, J., & Wang, B. (2014) Comparative study on seed germination character- istics of two species of Australia saltbush under salt stress. Acta Ecologica Sinica 34, 337-341. http://dx.doi.org/10.1016/j.chnaes.2013.07.011.

    [8] Dodd, G. L., & Donovan, L. A. (1999) Water potential and ionic effects on germination and seedling growth of two cold desert shrubs. American Journal of Botany 86, 1146-1153. http://dx.doi.org/10.2307/2656978.

    [9] Egli, D., & Rucker, M. (2012) Seed vigor and the uniformity of emergence of corn seedlings. Crop Science 52, 2774- 2782. http://dx.doi.org/10.2135/cropsci2012.01.0064.

    [10] Eneas-Filho, J., Oliveira-Neto, O., Prisco, J. T., Gomes-Filho, E., & Nogueira, C. M. (1995) Effects of salinity in vivo and in vitro on cotyledonary galactosidases from Vigna unguiculata (L.) Walp. during seed germination and seedling establishment. Revista Brasileira de Fisiologia Vegetal 7, 135-142.

    [11] Foolad, M., & Lin, G. (1997) Genetic potential for salt tolerance during germination in Lycopersicon species. Hort Science 32, 296-300.

    [12] Hampton, J. (2002) what is seed quality? Seed Science and Technology 30(1), 1-10.

    [13] Houle, G., Morel, L., Reynolds, C. E., & Siégel, J. (2001) The effect of salinity on different developmental stages of an endemic annual plant, Aster laurentianus (Asteraceae). American Journal of Botany 88, 62-67. http://dx.doi.org/10.2307/2657127.

    [14] Kandil, A., Sharief, A., Abido, W., & Ibrahim, M. (2012) Effect of salinity on seed germination and seedling characters of some forage sorghum cultivars. International Journal of Agriculture Sciences 4, 306-311. http://dx.doi.org/10.9735/0975-3710.4.7.306-311.

    [15] Katembe, W. J., Ungar, I. A., & Mitchell, J. P. (1998) Effect of salinity on germination and seedling growth of two Atriplex species (Chenopodiaceae). Annals of Botany 82, 167-175. http://dx.doi.org/10.1006/anbo.1998.0663.

    [16] Kaymakanova, M. (2009) Effect of salinity on germination and seed physiology in bean (Phaseolus vulgaris L.).

    [17] Biotechnology & Biotechnological Equipment 23, 326-329. http://dx.doi.org/10.1080/13102818.2009.10818430.

    [18] Kaydan, D., & Yagmur, M. (2008) Germination, seedling growth and relative water content of shoot in different seed sizes of triticale under osmotic stress of water and NaCl. African Joural Biotechnology 7, 2862-2868.

    [19] Kobayashi, H., Masaoka, Y., & Sato, S. (2005) Effects of excess magnesium on the growth and mineral content of rice and Echinochloa. Plant Production Science 8, 38-43. http://dx.doi.org/10.1626/pps.8.38.

    [20] Li, Y. (2008) Effect of salt stress on seed germination and seedling growth of three salinity plants. Pakistan Journal Biology Science 11, 1268-1272. http://dx.doi.org/10.3923/pjbs.2008.1268.1272.

    [21] Machado Neto, N. B., Saturnino, S. M., Bomfim, D. C., & Custódio, C. C. (2004) Water stress induced by mannitol and sodium chloride in soybean cultivars. Brazilian Archives of Biology and Technology 47, 521-529. http://dx.doi.org/10.1590/S1516-89132004000400004.

    [22] Mondo, V. H. V., Cicero, S. M., Dourado-Neto, D., Pupim, T. L., & Dias, M. A. N. (2013) Seed vigor and initial growth of corn crop. Journal of Seed Science 35, 64-69. http://dx.doi.org/10.1590/S2317-15372013000100009.

    [23] Panuccio, M., Jacobsen, S.-E., Akhtar, S., & Muscolo, A. (2014) Effect of saline water on seed germination and early seedling growth of the halophyte quinoa. AoB plants 6, 1-18. http://dx.doi.org/10.1093/aobpla/plu047.

    [24] Shainberg, I. (1975) Salinity of soils—Effects of salinity on the physics and chemistry of soils. In Plants in saline environments. Springer, pp. 39-55. http://dx.doi.org/10.1007/978-3-642-80929-3_4.

    [25] Shaul, O. (2002) Magnesium transport and function in plants: the tip of the iceberg. Biometals 15, 307-321. http://dx.doi.org/10.1023/A:1016091118585.

    [26] Van de Venter, A. (2001) What is seed vigour? Journal of New Seeds 2, 67-72. http://dx.doi.org/10.1300/J153v02n03_06.

    [27] Ramagopal, S. (1990) Inhibition of seed germination by salt and its subsequent effect on embryonic protein synnthesis in barley. Journal of Plant Physiology 136, 621-625. http://dx.doi.org/10.1016/S0176-1617(11)80224-5.

    [28] Tobe, K., Li, X., & Omasa, K. (2004) Effects of five different salts on seed germination and seedling growth of Haloxylon ammodendron (Chenopodiaceae). Seed Science Research 14, 345-353. http://dx.doi.org/10.1079/SSR2004188.

    [29] Tobe, K., Zhang, L., & Omasa, K. (2003) Alleviatory effects of calcium on the toxicity of sodium, potassium and

    [30] magnesium chlorides to seed germination in three non-halophytes. Seed Science Research 13, 47-54. http://dx.doi.org/10.1079/SSR2002123.

    [31] Ungar, I. (1995) Seed germination and seed-bank ecology in halophytes. In Seed development and germination (J. Kigel, Galili ed), Marcel Dekker, New York, pp. 599-628.

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How to Cite

Ghanad, M., Binit Nulit, R., Go, R., & Yong Seok Yien, C. (2016). The effects of NACL, KCL and MGCL2 on the germination of Brassica rapa var. parachinensis seed. International Journal of Biological Research, 4(1), 52-55. https://doi.org/10.14419/ijbr.v4i1.5854

Received date: February 9, 2016

Accepted date: March 1, 2016

Published date: April 19, 2016