Alterations of 8-iso-prostaglandin F2 alpha levels in heart failure patients with preserved ‎and reduced ejection fraction- a case-control study

Authors

  • Jasmine Chandra A

    PhD scholar, Department of Biochemistry, Sri Ramachandra Institute of Higher Education and ‎Research, Porur, Chennai, Tamil Nadu, India 600116 https://orcid.org/0000-0001-5224-5534

  • Sowmiya T

    Ph D Scholar, Department of Biochemistry, Sri Ramachandra Institute of Higher Education and ‎Research, Porur, Chennai, India 600116 https://orcid.org/0000-0001-8908-9432

  • Karthikeyan Rajamani

    Senior Scientist, Department of Public Health, Sri Ramachandra Institute of Higher Education ‎and Research, Porur, Chennai, Tamil Nadu, India https://orcid.org/0000-0001-6533-6689

  • Santhi Silambanan

    Professor of Biochemistry, Sri Ramachandra Institute of Higher Education and Research, ‎Porur, Chennai, Tamil Nadu, India, 600116

How to Cite

A, J. C., T, S., Rajamani, K. ., & Silambanan, S. (2025). Alterations of 8-iso-prostaglandin F2 alpha levels in heart failure patients with preserved ‎and reduced ejection fraction- a case-control study. International Journal of Basic and Applied Sciences, 14(1), 228-235. https://doi.org/10.14419/04d9k841

Received date: March 31, 2025

Accepted date: May 7, 2025

Published date: May 10, 2025

DOI:

https://doi.org/10.14419/04d9k841

Keywords:

Biomarkers; Cardiology; Heart Failure; Oxidative Stress; Stroke Volume

Abstract

Introduction: ‎Heart failure (HF) poses a global health challenge with decreased quality of life. HF can be HF ‎with reduced ejection fraction (HFrEF) or preserved ejection fraction (HFpEF). Oxidative ‎stress is implicated in the pathophysiology of heart failure. The study aimed to find the ‎association of the oxidative biomarker 8-iso-prostaglandin F2 Alpha (8-iso-PGF2α) with the ‎severity of heart failure. ‎

Methods:‎ The case-control study was conducted in the Departments of Cardiology and Biochemistry at ‎Sri Ramachandra Institute of Higher Education and Research, India. The study involved 80 HF ‎patients (HFpEF: n=40, HFrEF: n=40) aged between 30 and 75 years of both genders. ‎Enzyme-Linked Immunosorbent Assay analyzed 8-iso-PGF2α. Institutional ethics committee ‎approval was obtained. Statistical analysis was performed using SPSS software version 16. P ≤ ‎‎0.05 was considered statistically significant.‎

Results:‎ HFpEF was more prevalent in the 46-60 age group, whereas HFrEF was more common in the ‎‎61-75 age group. Among HFpEF and HFrEF, 8-iso-PGF2  levels were 910.07 ± 286.97 and ‎‎1185.02 ± 396.75 (p=0.001). 8-iso-PGF2 showed correlation with NT-proBNP and ‎echocardiography.‎

Conclusions: ‎Elevated 8-iso-PGF2α levels suggested a potential link between oxidative stress and heart ‎failure severity, contributing to our understanding of oxidative stress in heart failure

References

  1. McDonagh TA, Metra M, Adamo M, Gardner RS, Baumbach A, Böhm M, et al. 2021 ESC Guidelines for the diagnosis and treatment of acute and chronic heart failure: Developed by the Task Force for the diagnosis and treatment of acute and chronic heart failure of the European Society of Car-diology (ESC) With the special contribution of the Heart Failure Association (HFA) of the ESC. European heart journal. 2021 Sep 21;42(36):3599-726. https://doi.org/10.1093/eurheartj/ehab368.
  2. Yan T, Zhu S, Yin X, Xie C, Xue J, Zhu M, et al. Burden, trends, and inequalities of heart failure globally, 1990 to 2019: A secondary analysis based on the Global Burden of Disease 2019 study. J Am Heart Assoc 2023;12: e027852. https://doi.org/10.1161/JAHA.122.027852.
  3. Benjamin EJ, Muntner P, Alonso A, Bittencourt MS, Callaway CW, Carson AP, et al.; American Heart Association Council on Epidemiology and Prevention Statistics Committee and Stroke Statistics Subcommittee. Heart Disease and Stroke Statistics-2019 Update: A Report From the American Heart Association. Circulation. 2019 Mar 5;139(10): e56-e528. https://doi.org/10.1161/CIR.0000000000000659.
  4. Kalra A, Jose AP, Prabhakaran P, Kumar A, Agrawal A, Roy A, et al. The burgeoning cardiovascular disease epidemic in Indians - perspectives on contextual factors and potential solutions. Lancet Reg Health Southeast Asia. 2023 Feb 10;12:100156. https://doi.org/10.1016/j.lansea.2023.100156.
  5. Shi D, Tao Y, Wei L, Yan D, Liang H,Zhang J, et al. The burden of cardiovascular diseases attributed to diet high in sugar-sweetened beverages in 204 Countries and territories from 1990 to 2019, Current Problems in Cardiology. 2024; 49: 102043. https://doi.org/10.1016/j.cpcardiol.2023.102043.
  6. Golla MSG, Shams P. Heart Failure with Preserved Ejection Fraction (HFpEF) [Updated 2024 Mar 19]. In: StatPearls [Internet]. Treasure Island (FL): StatPearls Publishing; 2025 Jan-. Available from: https://www.ncbi.nlm.nih.gov/books/NBK599960/.
  7. Feng J, Zhang Y, Zhang J. Epidemiology and Burden of Heart Failure in Asia. JACC Asia. 2024 Mar 19;4(4):249-264. https://doi.org/10.1016/j.jacasi.2024.01.013.
  8. Huffman MD, Prabhakaran D. Heart failure: epidemiology and prevention in India. Natl Med J India. 2010 Sep-Oct;23(5):283-8. PMID: 21250584.
  9. Rotariu D, Babes EE, Tit DM, Moisi M, Bustea C, Stoicescu M, et al. Oxidative stress–Complex pathological issues concerning the hallmark of car-diovascular and metabolic disorders. Biomedicine & Pharmacotherapy. 2022 Aug 1;152:113238. https://doi.org/10.1016/j.biopha.2022.113238.
  10. Nishida M, Maruyama Y, Tanaka R, Kontani K, Nagao T, Kurose H. G alpha(i) and G alpha(o) are target proteins of reactive oxygen species. Nature. 2000 Nov 23;408(6811):492-5. https://doi.org/10.1038/35044120.
  11. Dhalla NS, Elimban V, Bartekova M, Adameova A. Involvement of Oxidative Stress in the Development of Subcellular Defects and Heart Disease. Biomedicines. 2022 Feb 7;10(2):393. https://doi.org/10.3390/biomedicines10020393.
  12. Urso C, Caimi G. Stress ossidativo e disfunzione endoteliale [Oxidative stress and endothelial dysfunction]. Minerva Med. 2011 Feb;102(1):59-77. Italian.
  13. Tsikas D, Suchy MT. Protocols for the measurement of the F2-isoprostane, 15 (S)-8-iso-prostaglandin F2α, in biological samples by GC–MS or GC–MS/MS coupled with immunoaffinity column chromatography. Journal of Chromatography B. 2016 Apr 15;1019:191-201. https://doi.org/10.1016/j.jchromb.2014.12.019.
  14. Geraci G, Sorce A, Zanoli L, Cuttone G, Calabrese V, Pallotti F, et al. Relationship Between 8-iso-prostaglandin-F2α and Predicted 10-Year Cardio-vascular Risk in Hypertensive Patients. Life. 2025; 15(3):401. https://doi.org/10.3390/life15030401
  15. Chaturvedi V, Parakh N, Seth S, Bhargava B, Ramakrishnan S, Roy A, et al. Heart failure in India: The INDUS (INDia Ukieri Study) study. J Pract Cardiovasc Sci 2016;2:28-35. https://doi.org/10.4103/2395-5414.182988.
  16. Prabhakaran D, Jeemon P, Roy A. Cardiovascular Diseases in India: Current Epidemiology and Future Directions. Circulation. 2016 Apr 19;133(16):1605-20. https://doi.org/10.1161/CIRCULATIONAHA.114.008729.
  17. Sreeniwas Kumar A, Sinha N. Cardiovascular disease in India: A 360 degree overview. Med J Armed Forces India. 2020 Jan;76(1):1-3. https://doi.org/10.1016/j.mjafi.2019.12.005.
  18. Ardeshna DR, Bob-Manuel T, Nanda A, Sharma A, Skelton WP 4th, Skelton M, Khouzam RN. Asian-Indians: a review of coronary artery disease in this understudied cohort in the United States. Ann Transl Med. 2018 Jan;6(1):12. https://doi.org/10.21037/atm.2017.10.18
  19. Izzo C, Vitillo P, Pietro PD, Visco V, Strianese A, Virtuoso N, et al. The Role of Oxidative Stress in Cardiovascular Aging and Cardiovascular Diseas-es. Life (Basel). 2021 Jan 15;11(1):60. https://doi.org/10.3390/life11010060.
  20. Regitz-Zagrosek V. Sex and Gender Differences in Heart Failure. Int J Heart Fail. 2020 Apr 13;2(3):157-181. https://doi.org/10.36628/ijhf.2020.0004
  21. Haymour L, Jean M, Smulski C, Legembre P. CD95 (Fas) and CD95L (FasL)-mediated non-canonical signaling pathways. Biochimica et Biophysica Acta (BBA)-Reviews on Cancer. 2023 Nov 1;1878(6):189004. https://doi.org/10.1016/j.bbcan.2023.189004.
  22. Bhullar SK, Shah AK, Dhalla NS. Role of Cardiomyocyte Apoptosis in Heart Failure. Biochemistry of Apoptosis and Autophagy. 2022:253-67. https://doi.org/10.1007/978-3-030-78799-8_14
  23. de Faria A, Modolo R, Moreno H. Plasma 8-Isoprostane as a Biomarker and Applications to Cardiovascular Disease. Biomarkers in Cardiovascular Disease. 2016;1:467-88. https://doi.org/10.1007/978-94-007-7741-5_31-1.
  24. Rababa'h AM, Guillory AN, Mustafa R, Hijjawi T. Oxidative Stress and Cardiac Remodeling: An Updated Edge. Curr Cardiol Rev. 2018 Mar 14;14(1):53-59. https://doi.org/10.2174/1573403X14666180111145207
  25. Bauer J, Ripperger A, Frantz S, Ergün S, Schwedhelm E, Benndorf RA. Pathophysiology of isoprostanes in the cardiovascular system: implications of isoprostane-mediated thromboxane A2 receptor activation. Br J Pharmacol. 2014 Jul;171(13):3115-31. https://doi.org/10.1111/bph.12677.
  26. Sawamura T, Kume N, Aoyama T, Moriwaki H, Hoshikawa H, Aiba Y, et al. An endothelial receptor for oxidized low-density lipoprotein. Nature. 1997 Mar 6;386(6620):73-7. https://doi.org/10.1038/386073a0.
  27. Wu J, Saleh MA, Kirabo A, Itani HA, Montaniel KR, Xiao L, et al. Immune activation caused by vascular oxidation promotes fibrosis and hyperten-sion. J Clin Invest. 2016 Jan;126(1):50-67. doi: 10.1172/JCI80761. Epub 2015 Nov 23. Erratum in: J Clin Invest. 2016 Apr 1;126(4):1607. https://doi.org/10.1172/JCI87425.
  28. Chrysohoou C, Konstantinou K, Tsioufis K. The Role of NT-proBNP Levels in the Diagnosis and Treatment of Heart Failure with Preserved Ejection Fraction-It Is Not Always a Hide-and-Seek Game. J Cardiovasc Dev Dis. 2024 Jul 16;11(7):225. https://doi.org/10.3390/jcdd11070225.
  29. Mallat Z, Philip I, Lebret M, Chatel D, Maclouf J, Tedgui A. Elevated levels of 8-iso-prostaglandin F2alpha in pericardial fluid of patients with heart failure: a potential role for in vivo oxidant stress in ventricular dilatation and progression to heart failure. Circulation. 1998 Apr 28;97(16):1536-9. https://doi.org/10.1161/01.CIR.97.16.1536.
  30. Kang HS, Park JJ, Choi JD et al. Prognostic value of NT-proBNP in heart failure with preserved versus reduced EF. Heart 2015;101:1881–8 https://doi.org/10.1136/heartjnl-2015-307782
  31. Kim JY, Lee JW, Youn YJ, Ahn MS, Ahn SG, Yoo BS, Lee SH, Yoon J, Choe KH. Urinary levels of 8-iso-prostaglandin f2α and 8-hydroxydeoxyguanine as markers of oxidative stress in patients with coronary artery disease. Korean Circ J. 2012 Sep;42(9):614-7. https://doi.org/10.4070/kcj.2012.42.9.614.

Downloads

How to Cite

A, J. C., T, S., Rajamani, K. ., & Silambanan, S. (2025). Alterations of 8-iso-prostaglandin F2 alpha levels in heart failure patients with preserved ‎and reduced ejection fraction- a case-control study. International Journal of Basic and Applied Sciences, 14(1), 228-235. https://doi.org/10.14419/04d9k841

Received date: March 31, 2025

Accepted date: May 7, 2025

Published date: May 10, 2025