In vitro callus induction of gerbera from leaf explant

Authors

  • Sadia Afrin Jui

    Bangladesh Jute Research Institute, Dhaka, Bangladesh

  • Md. Mijanur Rahman Rajib

    Bangabandhu Sheikh Mujibur Rahman Agricultural University

  • M. Mofazzal Hossain

    Bangabandhu Sheikh Mujibur Rahman Agricultural University

  • Sharmila Rani Mallik

    Bangladesh Agricultural Research Institute

  • Iffat Jahan Nur

    Bangladesh Jute Research Institute

  • Jannatul Ferdush

    Bangladesh Jute Research Institute

How to Cite

Afrin Jui, S., Mijanur Rahman Rajib, M., Mofazzal Hossain, M., Rani Mallik, S., Jahan Nur, I., & Ferdush, J. (2020). In vitro callus induction of gerbera from leaf explant. International Journal of Advanced Geosciences, 8(1), 1-4. https://doi.org/10.14419/ijag.v8i1.30000

Received date: October 20, 2019

Accepted date: November 30, 2019

Published date: January 11, 2020

DOI:

https://doi.org/10.14419/ijag.v8i1.30000

Keywords:

Callus, Gerbera, Growth Regulators, Leaf Explants, Culture.

Abstract

The experiment was designed to evaluate the effect of growth regulators on leaf explant of Gerbera for callus induction. Various kinds of plant growth regulators such as 6-Benzylaminopurine (BAP), α-Naphthalene acetic acid (NAA), 2, 4-Dichlorophenoxyacetic acid (2, 4-D), Indole-3-acetic acid (IAA) were used to initiate cultures. These were added to Murashige and Skoog medium in different combinations and concentrations. Leaf explants cultured on MS medium supplemented with BAP+ 2, 4-D+ IAA in T4 treatment & BAP+ 2,4-D in T5 treatment showed the best results for callus induction. On the other hand callus was induced early in the combination of BA+ 2,4-D + IAA hormone in T5, T9 & T8 treatment respectively. The rate of callus induction was very low in BA + NAA combinations but it was much earlier.

 

 

 

References

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How to Cite

Afrin Jui, S., Mijanur Rahman Rajib, M., Mofazzal Hossain, M., Rani Mallik, S., Jahan Nur, I., & Ferdush, J. (2020). In vitro callus induction of gerbera from leaf explant. International Journal of Advanced Geosciences, 8(1), 1-4. https://doi.org/10.14419/ijag.v8i1.30000

Received date: October 20, 2019

Accepted date: November 30, 2019

Published date: January 11, 2020